Microbe Bacteria DIOXIN Remediation

this was a @EPA and @DeptofDefense funded research project in Vietnam for Dioxin and Agent Orange remediation/soil reclamation. aka the EPA knows there are solutions avail to help in the #OhioChernobyl https://t.co/mMcCJWgnfg

— systembuster (@stlsystembuster) February 26, 2023

 Ohio Trainwreck Bioremediation Soil Treatment Research

JOINT STUDY OF BIOREMEDIATION AT PILOT SCALE FOR DETOXIFICATION OF HERBICIDE/DIOXIN IN DA NANG HOT SPOT, VIETNAM

Dang TCH1, Allen H2, Nguyen BH1, Fong V2, Dam TH1, Nguyen NQ1, Nguyen QH1, Phung KHC3, Dao TNA1 

Institute of Biotechnology, Vietnam Academy of Science and Technology (VAST);  

US Environment Protection Agency (EPA); Military Institute of Chemical and Environmental Research, MOD Vietnam

https://dioxin20xx.org/wp-content/uploads/pdfs/2010/10-1689.pdf

Introduction

Biodegradation of tetrachloro dibenzo-p-dioxin (TCDD) has been reported in the scientific literature, in the laboratory, and in pilot studies. From 1999 to 2009, Vietnamese researchers conducted several studies to detoxify heavily contaminated soil in the former Da Nang military base1

. 

Full-scale bioremediation of 3,384 m3 of dioxin contaminated soil was demonstrated in Bien Hoa, Vietnam, in 2009. Several international scientific work groups have concluded that bioremediation is the most environmentally responsible and cost-effective remedy for cleaning up Agent Orange residues at the former air bases in Vietnam.

More than 30 years after the US-Vietnam War, spilled Agent Orange defoliant solution containing traces of the dioxins, TCDD and octachloro dibenzo-p-dioxin (OCDD), 2,4,5-T, 2,4-D, and chlorophenols (TCP and DCP) remains in the soil and in lake sediment affected by contaminated soil, which had been carried by runoff from the former military airbase in Da Nang2 . Natural attenuation of the herbicides and dioxins has not been effective in detoxifying the soil or sediment. 

This first joint study by Vietnamese and American researchers was conducted to

demonstrate whether the soil in Da Nang can be bioremediated effectively using aerobic or anaerobic microbial processes. This study also sought to provide engineering design guidance to support the selection of either an aerobic or an anaerobic amendment recipe and an operating strategy to optimize biological treatment. 

Conclusions Aerobic bioremediation is capable of significantly reducing TCDD toxicity (p=0.0026). Bioaugmentation with small amounts of treated soil or contaminated sediment may be effective for anaerobic treatment. However, if suitable growth conditions are provided, the indigenous microbes in the mixed soil and sediment at Da Nang appear capable of degrading TCDD without adding another source of microbes. Anaerobic bioremediation rate is about half the rate of aerobic treatment, but the results are not as significant (p=0.25). From our of point active landfill containing both aerobic and anaerobic degradation become feasible resolution for detoxification of heavy herbicide/dioxin in full scale in Vietnam. 

Bioremediation is recognized as a “Green Technology,” which has a very low energy requirement and produces few emissions. Bioremediation is a permanent solution which produces a soil which can be returned to beneficial use. Knowledge gained from this project by both Vietnamese and US scientists will allow for design of customized recipes suitable for addressing dioxin and other persistent organic pollution problems throughout Vietnam and elsewhere 

@EPA Own Publications explained how to deal with #Ohio Chemical Incineration > U.S. Congress, Office of Technology Assessment, Dioxin Treatment Technologies Background Paper, OTA-BP-O-93 (Washington, DC: U.S. Government printing Office) pic.twitter.com/2VDdPloAYg

— systembuster (@stlsystembuster) February 26, 2023

This type of Incinerator is very similar to the #Downdraft #Gasifer Offgrid Hot Water and Hot Air system I designed and shared months ago. https://t.co/Im01RKt8uo pic.twitter.com/jGsSxS4aor

— systembuster (@stlsystembuster) February 26, 2023

#OhioChernobyl Research Files https://t.co/h1F8Khg5h6 to cleanup the #chemicals

This research paper from 1991 from the EPA CluIn Files for #Dioxin Incineration System provides a Diagram source file:
Dioxin Treatment Technologies
November 1991 OTA-BP-O-93 NTIS order PB92-152511 pic.twitter.com/VPsyW4ZqW1

— systembuster (@stlsystembuster) February 26, 2023

ElectroHemp 2019 Plans

2019 is the year ElectroHemp takes Phytoremediation Assisted Science to the Field in Pilot Study activities.
The Future Phytoremediation Assisted Soil and Water Cleanup Pilot Study Activity will include:

1. Business Partnerships
2. Job openings
3. Science and Scientific collaboration

ElectroHemp Green Remediation Intro

ElectroHemp Pilot Study's will be undertaken to perfect and streamline the organic green remediation system and process of the Team has been fine-tuning.
ElectroHemp Job Opportunity Examples: 9 or More job slots with possible Dual and Tri job responsibilities.

• Volunteers
• College Internships
• Hazmat Equipment Operator / Driver
• Greenhouse / Horticulturalists
• Environmental Scientist Laboratory & Testing Technician
• Mechanical + Equipment Mechanic
• Electronics Equipment Installation + maintenance
• Records and Bookkeeper 
• Attorney Environmental + Patents
• Spokesmen - Advertising 
• Marketing - Sales - Contracts

 If you are interested in joining the ElectroHemp team or have a property in the St.Louis Region you need assistance with we are interested in working with you.

ElectroHemp cleans soil and water with a Phytoremediation assisted process that turns pollution into cash.

Natural biofilters for toxic metals

The following Science Paper highlights how ElectroHemp Phytoremediation Rafts can be used as Biofilters to clean pollution from water sources.

Phytoremediation Raft Infographic- Plants cycle water toxins when grown on Rafts

a wide variety of agricultural and forestry by products have been used as biosorbents of toxic metals in a bid to develop biofilters for specific applications Electronic Journal of Biotechnology: 

The added benefit of how ElectroHemp equips these rafts with Electrokinetics will actually increase the toxic contamination removal because of the forced migration of the toxins is directed towards the rafts and plants roots which growing on the Phytoremediation Rafts.

ElectroHemp Phytoremediation Raft designs can be designed to remove any number or combination of toxic pollutants found in water sources.

 Real Life Example phytoremediation raft design for the removal of Lead (Pb) from Water

A floating phytoremediation raft constructed of: waste tea leaves, Pinus pinaster bark, Olea europea, Acacia nilotica bark. Which has these plant examples growing on it: Kenaf, Water Lettuce, Alligator Weed create a combination of Natural Solutions in the detoxification of Lead (Pb) from water. Scotty, ElectroHemp 

Phytoremediation Science Paper link

• i) Cotton - Hg; Groundnut skins - Cu; 
• Tree Bark (Pinus, Acacia etc.) - variety of metals; 
• Agrowaste - variery of metals; 
• waste tea leaves - Pb, Cd, and Zn; 
• Pinus radiata -U; 
• Apple waste -Variety of metals; 
• Cellulose - Variety of metals; Rice hulls - Variety of metals; 
• Exhausted coffee grounds - Hg; 
• Pinus pinaster bark - Zn, Cu, Pb. Saw mill dust (wood waste)- Cr; 
• Freshwater green algae - variety of metals; 
• Marine algae- Pb, Ni; 
• ii) Sphagnum (moss peat) - Cr(VI); 
• iii) Immobilized Aspergillus niger, A. oryzae - Cd, Cu, Pb, and Ni ; 
• Olive mill waste Olea europea Cr, Ni, Pb, Cd, and Zn, Cu and Ni; 
• Streptomyces rimosus (bacteria); 
• Saccharomyces cerevisiae (yeast); 
• Penicillium chrysogenum (fungi), Fuscus vesiculosus and Ascophyllum nodosum (marine algae) Zn, Cu andNi; Phanerochaete chrysosporium, P. versicolar - Pb, Ni, Cr, Cd, Cu; Pinus radiata - U;
• Immobilized Pseudomonas putida 5-X and Aspergillus niger, Mucor rouxxi - Cu; 
• Actionomycetes, Aspergillus niger, A.oryzae, Rhizopus arrhizus, R. nigricans- Cd; Rhizopus arrhizus - Cr(VI), Pb; Rhizopus nigricans, Phanarochaete chrysogenum -Pb; Aspergillus niger and Rhizopus arrhizus - Ni 

Acacia nilotica bark serves as an adsorbent of toxic metals. Bark (1 g) when added to 100 ml of aqueous solution containing 10 mg ml-1 metal solution exhibited different metal adsorption values for different metals. The order of metal adsorption being Cr > Ni > Cu > Cd> As > Pb. A similar trend of metal adsorption was observed when the bark is reused (1strecycle) Cr > Ni > Cu > Cd > Pb and also in the column-sorption. In order to verify the metal removal property of A. nilotica bark, toxicity bioassay with Salix viminalis stem cuttings in hydroponic system augmented with Cd, Cr and Pb together with A. nilotica bark powder was carried out. The results of toxicity bioassay confirmed the metal adsorption property of the bark powder. The functions of toxicity studies include leaf area, root length and number of new root primordia produced per stump. The leaf area, root length and number of new root primordia increased considerably in the presence of A. nilotica bark. The order of metal toxicity for leaf area and new root primordial is Cd > Cr > Pb. However, for root length the order of metal toxicity is Cr > Cd > Pb. The metal budgets of the leaf and root confirmed that the bark powder had adsorbed substantial amount of toxic metals and thus, alleviates the toxicity imposed by the various tested elements (Prasad et al. 2001).

Quercus ilex L. phytomass from stem, leaf and root as adsorbent of chromium, nickel, copper, cadmium and lead at ambient temperature was investigated. The metal uptake capacity of the root for different metals was found to be in the order of: Ni > Cd > Pb > Cu > Cr; stem Ni > Pb> Cu > Cd > Cr and leaf Ni > Cd > Cu > Pb > Cr. The highest amount adsorbed was Ni (root > leaf > stem). Data from this laboratory demonstrated that Ni is mostly sequestered in the roots where concentrations can be as high as 7.30 nmol/g dry weight, when one year old seedlings were treated with Ni (2000 mg/l) in pot culture experiments, compared to 0.13 nmol/g dry weight, in the control. This proves that the root biomass of Q. ilex has the capacity for complexing Ni. Chromium exhibited the least adsorption values for all the three types of phytomass compared to other metals. The trend of adsorption of the phytomass was similar for nickel and cadmium i.e. root > leaf > stem. Desorption with 10 mM Na2 EDTA was effective (55-90%). Hence, there exists the possibility of recycling the phytomass. The biosorption results of recycled phytomass suggests, that the selected adsorbents are reusable (Prasad and Freitas, 2000).

Phytoremediation EPA Field Research

Phytoremediation and prior EPA Field demonstrated projects to remediate heavy metals proves Bioremediation is a viable and cost saving option for Radianuclides removal.
The EPA has previously listed about 194 ongoing Phytoremediation / bioremediation field research projects. Yr 2000

194 ongoing phytoremediation field research projects, EPA 

Heavy metals and radionuclides represent about 30% of this activity supporting that bioremediation is a feasible technology to decontaminate the environment. 

Unlike many organic contaminants most:

•  metals and radionuclides cannot be eliminated from the environment by chemical or biological transformation. 
• Although it may be possible to reduce the toxicity of certain metals by influencing their speciation, 
• they do not degrade and are persistent in the environment. 

The conventional remediation technologies that are used to clean heavy metal polluted environments are:

• soil in situ vitrification
• soil incineration
• excavation and landfill
• soil washing
• soil flushing
• solidification
• stabilization with electrokinetic systems 

Source: Electronic Journal of Biotechnology

ElectroHemp is entering: Innovating Soil 3.0 Contest

It's official, ElectroHemp has entered the FoodShot Global  contest! 

Announcement middle January

ElectroHemp has entered the FoodShot Global  contest! 

Have you heard about the 2018-2019 FoodShot Global event that will be promoting soil health to increase food safety? 

ElectroHemp will be entering the process and system that organically cleans soil and water, better known as green remediation in the FoodShot’s first annual challenge — Innovating Soil 3.0. 

ElectroHemp will be competing and in the running for up to $10 million in equity funding and $20 million in debt funding!!!

Rajiv Singh, co-chairman of FoodShot Global, wrote on Agfunder about the contest: a search for projects and ideas that utilize the latest in technology, science, and engineering to address the crisis of soil deterioration. In particular, he writes about how a diverse set of technologies can be brought to bear to improve the conditions of the world’s soils, from machinery and equipment to low-tech farming practices, to carbon sequestration tools.

FoodShot Global Our Story 

Why is the food we eat making us sick? Why is good food more expensive? Why is there growing hunger and malnutrition in a world of plenty? Why does farming pollute and degrade our land, water, and air? 
There is a simple answer: the food system is broken. So let’s fix it. There is a better way. 
FoodShot is a collaborative platform of innovators, investors, industry leaders, and advocates who are working together to solve our biggest food system challenges.   By connecting knowledge, networks, and funding across the private and public sectors, FoodShot creates scalable, impactful, and inspired solutions – MoonShots For Better Food. 
With a global network of food industry leaders and aligned equity, debt, and prize dollars; FoodShot empowers best-in-class entrepreneurs, researchers, and advocates to transform the most critical food system challenges into opportunities for collaborative investment and meaningful change.
PROGRAM AND PROCESS  
On an annual basis, FoodShot will undertake the following,
DEFINE an annual FoodShot challenge. 
SEARCH for ground-breaking FoodShot entrepreneurs, researchers, and advocates.
VET submissions according to defined criteria.
AWARD aligned equity, debt, and non-dilutive capital to world-class ideas and businesses that are scalable, impactful, and inspired solutions to the challenge.
SCALE by leveraging a global network of stakeholders in food and agriculture. 
ECOSYSTEM DEVELOPMENT of key thought-leaders in innovation, investment, industry and policy.  
Learn more about the Food Shot Global Opportunities at their Facebook Page Foodshot Global Facebook Page
And while you are on Facebook feel free to drop by the Hemp Environmental Forum Facebook page where Scotty also shares information about the ElectroHemp system and process and wish us luck!