Uranium Water Biofilter Remediation

ElectroHemp blog post on Uranium Reducing Phytoremediation Raft Design

ElectroHemp Phytoremediation Raft designs can be designed to remove any number or combination of toxic pollutants found in water sources

Previously ElectroHemp highlighted how Natural biofilters for toxic metals can be used for Pb (Lead) Removal. This same technique can be used for Uranium (U) removal. 
All that needs to be done is substitute the Raft and Plants that will extract Uranium and it's by products.
Example: A phytoremediation raft can be constructed with these biosorbing products: Tree Bark (Pinus, Acacia), Agro Wastes (Tea Leaves, Rice Hulls) Apple Wastes . With these type of hyperaccumulating plant species: Hemp, Kenaf, Sun Flowers, Mustard Grass, Rape, even some Grasses 
To ensure all the Toxic Contamination comes in contact with the Raft and Plant Roots growing on the Phytoremediation Rafts that phytoextract the toxins. ElectroHemps uses Electrokinetics into the Remediation removal process. Electrokinetics draws toxins where directed.

ElectroHemps combines Electrokinetics, Phytoremediation, and Biofilters into the Remediation removal process. Key point: Electrokinetics draws toxins where directed.

Natural biofilters for toxic metals

The following Science Paper highlights how ElectroHemp Phytoremediation Rafts can be used as Biofilters to clean pollution from water sources.

Phytoremediation Raft Infographic- Plants cycle water toxins when grown on Rafts

a wide variety of agricultural and forestry by products have been used as biosorbents of toxic metals in a bid to develop biofilters for specific applications Electronic Journal of Biotechnology: 

The added benefit of how ElectroHemp equips these rafts with Electrokinetics will actually increase the toxic contamination removal because of the forced migration of the toxins is directed towards the rafts and plants roots which growing on the Phytoremediation Rafts.

ElectroHemp Phytoremediation Raft designs can be designed to remove any number or combination of toxic pollutants found in water sources.

 Real Life Example phytoremediation raft design for the removal of Lead (Pb) from Water

A floating phytoremediation raft constructed of: waste tea leaves, Pinus pinaster bark, Olea europea, Acacia nilotica bark. Which has these plant examples growing on it: Kenaf, Water Lettuce, Alligator Weed create a combination of Natural Solutions in the detoxification of Lead (Pb) from water. Scotty, ElectroHemp 

Phytoremediation Science Paper link

• i) Cotton - Hg; Groundnut skins - Cu; 
• Tree Bark (Pinus, Acacia etc.) - variety of metals; 
• Agrowaste - variery of metals; 
• waste tea leaves - Pb, Cd, and Zn; 
• Pinus radiata -U; 
• Apple waste -Variety of metals; 
• Cellulose - Variety of metals; Rice hulls - Variety of metals; 
• Exhausted coffee grounds - Hg; 
• Pinus pinaster bark - Zn, Cu, Pb. Saw mill dust (wood waste)- Cr; 
• Freshwater green algae - variety of metals; 
• Marine algae- Pb, Ni; 
• ii) Sphagnum (moss peat) - Cr(VI); 
• iii) Immobilized Aspergillus niger, A. oryzae - Cd, Cu, Pb, and Ni ; 
• Olive mill waste Olea europea Cr, Ni, Pb, Cd, and Zn, Cu and Ni; 
• Streptomyces rimosus (bacteria); 
• Saccharomyces cerevisiae (yeast); 
• Penicillium chrysogenum (fungi), Fuscus vesiculosus and Ascophyllum nodosum (marine algae) Zn, Cu andNi; Phanerochaete chrysosporium, P. versicolar - Pb, Ni, Cr, Cd, Cu; Pinus radiata - U;
• Immobilized Pseudomonas putida 5-X and Aspergillus niger, Mucor rouxxi - Cu; 
• Actionomycetes, Aspergillus niger, A.oryzae, Rhizopus arrhizus, R. nigricans- Cd; Rhizopus arrhizus - Cr(VI), Pb; Rhizopus nigricans, Phanarochaete chrysogenum -Pb; Aspergillus niger and Rhizopus arrhizus - Ni 

Acacia nilotica bark serves as an adsorbent of toxic metals. Bark (1 g) when added to 100 ml of aqueous solution containing 10 mg ml-1 metal solution exhibited different metal adsorption values for different metals. The order of metal adsorption being Cr > Ni > Cu > Cd> As > Pb. A similar trend of metal adsorption was observed when the bark is reused (1strecycle) Cr > Ni > Cu > Cd > Pb and also in the column-sorption. In order to verify the metal removal property of A. nilotica bark, toxicity bioassay with Salix viminalis stem cuttings in hydroponic system augmented with Cd, Cr and Pb together with A. nilotica bark powder was carried out. The results of toxicity bioassay confirmed the metal adsorption property of the bark powder. The functions of toxicity studies include leaf area, root length and number of new root primordia produced per stump. The leaf area, root length and number of new root primordia increased considerably in the presence of A. nilotica bark. The order of metal toxicity for leaf area and new root primordial is Cd > Cr > Pb. However, for root length the order of metal toxicity is Cr > Cd > Pb. The metal budgets of the leaf and root confirmed that the bark powder had adsorbed substantial amount of toxic metals and thus, alleviates the toxicity imposed by the various tested elements (Prasad et al. 2001).

Quercus ilex L. phytomass from stem, leaf and root as adsorbent of chromium, nickel, copper, cadmium and lead at ambient temperature was investigated. The metal uptake capacity of the root for different metals was found to be in the order of: Ni > Cd > Pb > Cu > Cr; stem Ni > Pb> Cu > Cd > Cr and leaf Ni > Cd > Cu > Pb > Cr. The highest amount adsorbed was Ni (root > leaf > stem). Data from this laboratory demonstrated that Ni is mostly sequestered in the roots where concentrations can be as high as 7.30 nmol/g dry weight, when one year old seedlings were treated with Ni (2000 mg/l) in pot culture experiments, compared to 0.13 nmol/g dry weight, in the control. This proves that the root biomass of Q. ilex has the capacity for complexing Ni. Chromium exhibited the least adsorption values for all the three types of phytomass compared to other metals. The trend of adsorption of the phytomass was similar for nickel and cadmium i.e. root > leaf > stem. Desorption with 10 mM Na2 EDTA was effective (55-90%). Hence, there exists the possibility of recycling the phytomass. The biosorption results of recycled phytomass suggests, that the selected adsorbents are reusable (Prasad and Freitas, 2000).

Toxic Contamination and Remediation

The design and process of the ElectroHemp BioRad Toxic Contamination and Remediation system performs the steps addressed in this Phytoremediation Science Paper without having to Genetically Alter anything. 

Accomplishing 3 of the 4 needed steps mentioned below.

Figure 4. Several factors would accelerate phytoremediation technology. The prime being: genetic engineering and production of transgenics having tolerance and metal accumulation ability for use in phytoremediation, facilitating the factors that would influence the metal bioaccumulation coefficient which inturn will depends upon heavy metal availability in the soil, absorption, transport and sequestration etc, and development of low cost technologies for chelate-induced hyperaccumulation.

Phytoremediation EPA Field Research

Phytoremediation and prior EPA Field demonstrated projects to remediate heavy metals proves Bioremediation is a viable and cost saving option for Radianuclides removal.
The EPA has previously listed about 194 ongoing Phytoremediation / bioremediation field research projects. Yr 2000

194 ongoing phytoremediation field research projects, EPA 

Heavy metals and radionuclides represent about 30% of this activity supporting that bioremediation is a feasible technology to decontaminate the environment. 

Unlike many organic contaminants most:

•  metals and radionuclides cannot be eliminated from the environment by chemical or biological transformation. 
• Although it may be possible to reduce the toxicity of certain metals by influencing their speciation, 
• they do not degrade and are persistent in the environment. 

The conventional remediation technologies that are used to clean heavy metal polluted environments are:

• soil in situ vitrification
• soil incineration
• excavation and landfill
• soil washing
• soil flushing
• solidification
• stabilization with electrokinetic systems 

Source: Electronic Journal of Biotechnology

Phytoremediation Alligator Weed Lead + Mercury

Alligator Weed (Alternanthera philoxeroides) was used for removal of lead and mercury from polluted waters. It is possible to use these species to restore the biosolid and sewage sludge contaminated sites, while exercising caution on human consumption.

Phytoremediation with Alligator Weed to remove Lead and Mercury from water.

Alternanthera philoxeroides, commonly referred to as alligator weed, is a native species to the temperate regions of South America, which includes Argentina, Brazil, Paraguay and Uruguay. Argentina alone, hosts around 27 species that fall within the range of the genus Alternanthera. Wikipedia

Article Science paper: Metal hyperaccumulation in plants - Biodiversity prospecting for phytoremediation technologys source Edible plants and vegetables crops plants and vegetables crops

The dominant leaf vegetable producing species viz. Amaranthus spinosus, Alternanthera philoxeroides and A. sessiles growing on the sewage sludge of Musi river located in greater Hyderabad City (close to 17º26' N latitude and 78º27' E longitude), Andhra Pradesh, India was investigated for metal accumulation. 

• The transfer factor for metals was calculated Metal content in plant part (dry wt.)/ Metal content in soil (dry.wt).
• Transfer factor and metal content Cd (non-essential), Zn and Fe (essential) in plant parts of these selected species indicate their aility to bioconcentrate in their tissues (Figure 12). 
• The concentration of these metals is invariably high in leaf tissue (Bañuelos and Meek, 1989; Prasad, 2001b). 
• Thus, it is possible to use these species to restore the biosolid and sewage sludge contaminated sites, while exercising caution on human consumption. 
  

 It is also possible to supplement the dietary requirement of human food with Zn and Fe as these being essential nutrients and the plant species are edible. 

[Warning] However, there is a need to monitor the metal transfer factor through food chain (Bañuelos and Meek, 1989; Bañuelos et al. 1993a; Bañuelos et al. 1993b).

Alligator Weed description courtesy of Wikipedia- Alternanthera philoxeroides can thrive in both dry and aquatic environments and is characterized by whitish, papery flowers along its short stalks, irregular, or sprawling hollow stems, and simple and opposite leave pattern sprouting from its nodes. The species is dioecious. It is also considered a herbaceous plant due to its short-lived shoot system. It produces horizontal stems, otherwise known as stolons, that can sprout up to 10 m in length and thanks to its hollow stems, floats easily. This results in large clusters of stem to amass and create dense mats along the surface. The plant flowers from December to April and usually grows around 13 mm in diameter and tend to be papery and ball-shaped. The weed's intricate root system can either allow them to hang free in the water to absorb nutrients or directly penetrate the soil/sediment and pull their nutrients from below.

Hemp PFAS groundwater remediation

Too bad the EPA Scientist weren't interested in Solving the Pollution that is contaminating this earth. https://t.co/Kp1JDyrJKq https://t.co/estfCyq4uY

— systembuster (@stlsystembuster) November 14, 2022

Researcher Dr Brett Turner from Newcastle University and his team have developed a natural and effective solution for removing toxic PFAS chemicals from groundwater.

Episode Notes

Researcher Dr Brett Turner from Newcastle University and his team have developed a natural and effective solution for removing toxic PFAS chemicals from groundwater.

 

He also explains how their research is also looking at how hemp plants can be used to remediate PFAS contaminated soil. 

University of Newcastle researchers are on track to create a solution to per-and poly- fluoroalkyl substances (PFAS) contamination, in a project that could benefit the Williamtown community and countless other sites across the world. 

L to R: Research team members Mr Glenn Currell, Dr Brett Turner and Dr Dan Bishop.

Dr Brett Turner and a team from the University’s Priority Research Centre for Geotechnical Science and Engineering, are to continue investigating the use of hemp seed proteins, and the hemp plant itself, to treat water and soil contaminated with PFAS. As announced in the 2019-2020 budget, the Federal Government has awarded $4.7m to the researchers for this work.

The man-made chemicals known as PFASs have been widely used in food wrappers, textile stains, non-stick cooking utensils, carpet and furniture protectants, insecticides, electronics, and in fire-fighting foams, as they are highly effective against hydrocarbon fuel fires.

Within Australia a number of sites have been identified as having groundwater and soil contaminated with PFAS including the Williamtown RAAF base in NSW; Oakey Aviation Centre in QLD, and the Country Fire Authority (CFA) training facility in Fiskville VIC.

Globally, the extent of this problem is even greater, with more than 41,000 airports in the world, many of which are potentially contaminated with PFAS. Considered almost non-degradable in nature, many conventional treatments for PFAS remediation are not effective, yet the costs of PFAS remediation technologies are exorbitant.

Dr Turner said the team’s early findings, supported by an initial $600,000 grant through the NSW Government’s Research Attraction and Acceleration Program, were being further explored, and applied to the more complex challenge of contaminated soil.

“We found that hemp has a remarkable affinity for PFAS chemicals in groundwater, so we expect that this can be applied to remediate contaminated soil – an area where currently there are no options,” Dr Turner said.

Director of the Priority Research Centre and 2015 NSW Scientist of the Year, Laureate Professor Scott Sloan said the next stage of the research would pioneer a more cost-effective way of removing chemical compounds from soil, groundwater and surface waters in a natural way.

“We are excited about the potential benefits for the residents around our local RAAF base at Williamtown, and for other affected communities worldwide,” Laureate Professor Sloan said.

The $4.7m funding has been awarded through the Department of Industry, Innovation and Science over five years. This research is also supported by the University of Newcastle with additional funding of $1.5m.

“We’d like to thank the Government for the funding, as well as Senator Brian Burston for his significant efforts in helping to secure it,” Dr Brett Turner said.

“This critical grant will allow us to increase our team, employing three Doctoral Fellows, four PhD students and a research technician. We look forward to continuing the hard work, and pioneering a local solution to a global problem.”

Here is the audio recording see photos below of the system Scotty with ElectoHemp has been designing and working on.

RXleaf- Hemp Cleans Up Radioactive Soil

It's great that RX leaf highlights the value of using Hemp to clean up Radioactive Soil in their Article

Many people get this. But don't realize: 
1) what is the next step? What's a person do with a bunch of toxic plants? 
2) what about the places in the soil that the hemp roots don't come in contact with the toxins? Meaning the toxins are still there. 
3) 99% of any science papers all say: phytoremediation takes a long time. Who wants to wait, when the toxins need removed asap? 
4) shouldn't all birds, animals, and humans be kept away from toxic Hemp plants? Fences don't stop birds, animals, or people. 
5) lots of plants can be used for phytoremediation (400 that I'm aware of). Is it better to have a huge plant to dispose of with X amount of toxins or a smaller plant with the same X amount of toxins? ....

I agree Hemp is great, and is even greater when used in combination with the system our team has figured out that both speeds up the toxic removal and also disposes it safely eliminating all the hazards that so many don't realize.

It's not rocket science, it's phyto science. And the #ElectroHemp system also turns the hazardous waste infected plants into cash!
Learn more at the https://m.facebook.com/HempEnvironmentalForum/ or the blogs: #ElectroHemp and #MOHempEnergy

Reduction of Uranium

...the reduction of uranium was done by quantifying the fraction of uranium in both the soluble and insoluble pools. 

• Uranium in the cultures spiked to 3 μM shifted from ~90% soluble at the T0 time point for all 3 concentrations to 70–97% insoluble by the end of the 24 day incubations (Fig 3). 
• A mass balance indicated that 93–102% of the added uranium could be accounted for in the soluble/insoluble pools. 
• Interestingly, the bacterium also reduced uranium as efficiently at 5 and 10 μM concentration (up to 97%-S2 Fig). 
• However, the percent of soluble uranium at the T0 sampling time point was 78% for the 5 μM and 18% for the 10 μM treatments. Presumably, the bacteria were stimulated by the prior exposure to uranium during transfer and began reducing the radionuclide before the T0 samples could be collected. link
  

Electrokinetic coupled Phytotechnology - ElectroHemp

3 Electrokinetic coupled Phytotechnology articles that support the ElectroHemp phytoremediation techniques the team has developed. All Science Papers on Phytoremediation say, " Its Slow " < Therefore to overcome this obstacle and go above and beyond this fault. It only makes sense to speed up things up with something I'm sure Nicola Tesla would approve of with Electrokinetics system that sends low voltage pulses thru water or soil and at the same time helps plants grow bigger faster with higher harvest results. BOOM.

79 Research Articles on Phytoremediation for Bioenergy

its not Rocket Science its Phyto Science!  79 Research Articles used as a Reference in this Science paper by Helena Gomes.

Phytoremediation for bioenergy: challenges and opportunities

Helena I. Gomes 

Pages 59-66 | Received 23 Oct 2011, Accepted 20 May 2012, Accepted author version posted online: 24 May 2012, Published online: 25 Jun 2012

• Download citation
 
• https://doi.org/10.1080/09593330.2012.696715
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